The Confederation of African Football (Caf) took action after a complaint from Tanzanian side Simba about the eligibility of Janvier Besala Bokungu.
Mazembe beat Simba 6-3 on aggregate in the second round of the tournament.
Simba will play Morocco’s Wydad Casablanca, who lost to Mazembe in the third round, for a place in the group stages of the tournament.
The one-off game will be played at a neutral venue next week.
Mazembe have won the Champions League for the last two years and reached the final of the Club World Cup last season, losing 3-0 to Italians Inter Milan in the final.
Bokungu, a 22-year-old Kinshasa-born defender, was transferred from Mazembe to Tunisian club Esperance in 2007 and rejoined the Congolese club this year.
Reports say he then broke a contract with the Tunis team that runs until June this year, but a Mazembe official insisted the Bokungu transfer was legal.
“Janvier properly revoked his contract with Esperance and Mazembe have no problem with the player or his former club. He was acquired after a mutual agreement,” Mazembe general manager Frederic Kitengie told reporters.
Mazembe are the African Champions League holders
Reigning African champions TP Mazembe of DR Congo have been disqualified from the Champions League.
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